| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Butyricimonas synergistica | TACATTCCGGATGCCCTGC | TGCGCAAGTGTCGTCCAT | 59.85 | 59.97 | 225 | 149 |
100.00%
|
100.00%
|
| Primer 2 | Butyricimonas synergistica | AGCCGACCCAAGCAAACT | TGCGCAAGTGTCGTCCAT | 59.48 | 59.97 | 184 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Butyricimonas synergistica | CTCTGCTGCTGATGAGGCA | ACCGTCATTCCATGCGCA | 59.78 | 60.05 | 258 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Butyricimonas synergistica | TCTGCTGCTGATGAGGCA | ACCGTCATTCCATGCGCA | 58.60 | 60.05 | 257 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Butyricimonas synergistica | TCTCTGCTGCTGATGAGGC | ACCGTCATTCCATGCGCA | 59.48 | 60.05 | 259 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Butyricimonas synergistica | TAGGTGCTTGTGTCGGAGC | TCGCGAAAAGGGCAACAC | 59.71 | 58.97 | 171 | 136 |
100.00%
|
100.00%
|
| Primer 7 | Butyricimonas synergistica | AGCCGAAAGAGCCGAAGT | TCCAACCACGCAAAGCCA | 58.94 | 60.12 | 190 | 135 |
100.00%
|
100.00%
|
| Primer 8 | Butyricimonas synergistica | AGCCGAAAGAGCCGAAGTC | TCCAACCACGCAAAGCCA | 60.08 | 60.12 | 190 | 135 |
100.00%
|
100.00%
|
| Primer 9 | Butyricimonas synergistica | TTACGCAGTGGTGGGGTTG | TCCAACCACGCAAAGCCA | 60.23 | 60.12 | 168 | 135 |
100.00%
|
100.00%
|
| Primer 10 | Butyricimonas synergistica | CCGAAAGAGCCGAAGTCCA | TCCAACCACGCAAAGCCA | 59.71 | 60.12 | 188 | 135 |
100.00%
|
100.00%
|